AF485783 14758 double DNA circular SYN 15-MAY-2003 21-MAR-2002 Binary vector pBI121, complete sequence AF485783 AF485783.1 gb|AF485783.1| gi|19569229 Binary vector pBI121 Binary vector pBI121 other sequences; artificial sequences; vectors 1 1..14758 Chen,P.Y. Wang,C.K. Soong,S.C. To,K.Y. Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants Mol. Breed. 11, 287-293 (2003) 2 1..14758 To,K.Y. Direct Submission Submitted (20-FEB-2002) Institute of BioAgricultural Sciences, Academia Sinica, Taipei 11529, Taiwan source 1..14758 1 14758 AF485783.1 organism Binary vector pBI121 mol_type genomic DNA db_xref taxon:189807 note constructed using pB221 from Clontech Laboratories and Bin19 described in GenBank Accession Number U09365 misc_feature complement(13..796) 796 13 AF485783.1 note similar to traF in GenBank Accession Number X54459 rep_origin complement(790..1168) 1168 790 AF485783.1 note ColE1 ori; similar to sequence in GenBank Accession Number V00268 misc_feature complement(1161..2344) 2344 1161 AF485783.1 note similar to tetA in GenBank Accession Number X75761 misc_feature complement(2454..2478) 2478 2454 AF485783.1 note T-DNA right border promoter 2519..2825 2519 2825 AF485783.1 note NOS gene 2838..3632 2838 3632 AF485783.1 gene nptII CDS 2838..3632 2838 3632 AF485783.1 gene nptII codon_start 1 transl_table 1 product neomycin phosphotransferase II protein_id AAL92039.1 db_xref GI:19569230 translation MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF terminator 4022..4277 4022 4277 AF485783.1 note NOS promoter 4974..5808 4974 5808 AF485783.1 note CaMV 35S gene 5845..7656 5845 7656 AF485783.1 gene gusA CDS 5845..7656 5845 7656 AF485783.1 gene gusA note GUS codon_start 1 transl_table 1 product beta-glucuronidase protein_id AAL92040.1 db_xref GI:19569231 translation MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ terminator 7727..7979 7727 7979 AF485783.1 note NOS misc_feature complement(8621..8646) 8646 8621 AF485783.1 note T-DNA left border misc_feature complement(9156..10198) 10198 9156 AF485783.1 note similar to tetA in GenBank Accession Number L13842 misc_feature complement(10199..11680) 11680 10199 AF485783.1 note similar to trfA in GenBank Accession Number X00713 misc_feature complement(11681..12673) 12673 11681 AF485783.1 note similar to NPTIII gene in GenBank Accession Number V01547 misc_feature complement(12674..13443) 13443 12674 AF485783.1 note similar to transposable element IS1 in GenBank Accession Number X58999 misc_feature complement(13444..13794) 13794 13444 AF485783.1 note similarity to NPT III gene in GenBank Accession Number V01547 misc_feature complement(13795..14066) 14066 13795 AF485783.1 note similar to kilA in GenBank Accession Number M62846 rep_origin complement(14141..14758) 14758 14141 AF485783.1 note ori V; similar to sequence in GenBank Accession Number M20134 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